|
KMID : 0903520010440040219
|
|
Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2001 Volume.44 No. 4 p.219 ~ p.223
|
|
|
Conditions of Hemoglobin Hydrolysis and Separation for the production of Enriched Heme - iron
|
|
Oh Nam-Soon
|
|
|
Abstract
|
|
|
|
Effects of hemoglobin (Hgb) concentration and degree of hydrolysis (DH) of Hgb on the separation of heme-iron were examined to produce highly enriched heme-iron from Hgb hydrolysate. Separation efficiency of Hgb hydrolysate with different DH was studied at wide pH range (pH 1.0∼11.0). Separation efficiency expressed as heme-iron/peptide ratio increased with decreasing Hgb concentration. When 5% Hgb (pH 10.0) was hydrolyzed using commercially available Esperase for 5 h at 50℃, DH was 25%. The precipitation of heme-iron-enriched peptides were remarkably high at pH range 3∼6. Optimal pH range for heme-iron with high heme-iron/peptide ratio shifted to acidic pH with increasing DHs of Hgb. The enriched heme-iron fraction in the precipitates showed a single band through urea-SDS-PAGE, with a molecular mass of 1 kDa. In the dry heme-iron product produced in a pilot bioreactor, content of heme-iron and heme-iron/peptide ratio were 27.1 and 38.7%, respectively, and production yield was 9.3%.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|